V4888
Sigma
Anti-VSV-G antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Vesicular Stomatitis Virus glycoprotein |
| MDL number: | MFCD00164761 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C) of the Vesicular Stomatitis Virus glycoprotein (VSV-G), conjugated to maleimide activated KLH via a cysteine residue added at the carboxyl terminus. The antibody recognizes the VSV-G tag at both the C-terminus and N-terminus. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| species reactivity | mammals |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates |
| immunoprecipitation: 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates | |
| indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
